Tang, Lichun et al. 2017
CRISPR/Cas9-mediated gene editing in human zygotes using Cas9 protein
Previous works using human tripronuclear zygotes suggested that the clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 system could be a tool in correcting disease-causing mutations. However, whether this system was applicable in normal human (dual pronuclear, 2PN) zygotes was unclear. Here we demonstrate that CRISPR/Cas9 is also effective as a gene-editing tool in human 2PN zygotes. By injection of Cas9 protein complexed with the appropriate sgRNAs and homology donors into one-cell human embryos, we demonstrated efficient homologous recombination-mediated correction of point mutations in HBB and G6PD. However, our results also reveal limitations of this correction procedure and highlight the need for further research.
Gwern Branwen’s comments:
Even nicer: another human-embryo CRISPR paper. Some old 2015 work – results: no off-target mutations and efficiencies of 20/50/100% for various edits. (As I predicted, the older papers, Liang et al 2015 / Kang et al 2016 / Komor et al 2016, were not state of the art and would be improved on considerably.)
Back in February 2015, qualia researcher Mike Johnson predicted that dedicated billionaire with scant regard for legalistic regulations could start genetically “spellchecking” their offspring within 5-7 years.
But if anything, he might have overestimated the timeframe.